Table 2.
Neospora caninum-recombinant antigens and cross-reactions tested against Toxoplasma gondii and related protozoan parasites
| N. caninum-recombinant antigens established for diagnostic purposes (other names or variants of the recombinant proteins) | Localisation (estimated size, reference) | Parasite species against which cross-reactivity was tested | References which examined cross-reactivity |
|---|---|---|---|
| rNcSAG1 (rNcp29, NcSAG1t) | Surface (29–38 kDa) (Howe et al. 1998; Sonda et al. 1998) | T. gondii | (Howe et al. 2002; Chahan et al. 2003; Liao et al. 2005b; Huang et al. 2007; Ybanez et al. 2013) |
| Sarcocystis spp. | (Howe et al. 2002) | ||
| rNcSRS2 (Ba/SRS2p44, NcSRS2t, tNcSRS2) | Surface (35–43 kDa) (Hemphill and Gottstein, 1996; Hemphill et al. 1997; Howe et al. 1998) | T. gondii | (Nishikawa et al. 2001, 2002; Gaturaga et al. 2005; Huang et al. 2007; Liu et al. 2007) |
| H. heydorni | (Nishikawa et al. 2001, 2002) | ||
| rNcSAG4 | Surface (18 kDa) (Fernandez-Garcia et al. 2006) | T. gondii | (Aguado-Martinez et al. 2008) |
| B. besnoiti | (Aguado-Martinez et al. 2008) | ||
| Sarcocystis spp. | (Aguado-Martinez et al. 2008) | ||
| rNcGRA6 (Nc14·1, NcDG2, NcGRA6d, NcGRA6s) | Dense granules (68 kDa, 37 kDa) (Liddell et al. 1998) | T. gondii | (Lally et al. 1996; Jenkins et al. 1997) |
| Sarcocystis spp. | (Lally et al. 1996; Jenkins et al. 1997) | ||
| rNcGRA7 (Nc4·1, NcDG1, N57, MBPNcGRA7, rNcGRA7t, rNcGRA7) | Dense granules (33 kDa, 17–18 kDa) (Lally et al. 1997; Hemphill et al. 1998; (Alvarez-Garcia et al. 2007; Fuchs et al. 1998) | T. gondii | (Lally et al. 1996; Jenkins et al. 1997; Aguado-Martinez et al. 2008; Hamidinejat et al. 2015) |
| B. besnoiti | (Aguado-Martinez et al. 2008) | ||
| Sarcocystis. spp. | (Lally et al. 1996; Jenkins et al. 1997; Aguado-Martinez et al. 2008) | ||
| Others, including Babesia spp., Tritrichomonas fetus, Leishmania infantum | (Louie et al. 1997; Hara et al. 2006; Huang et al. 2007) | ||
| rNcMIC10 (NcMiC10 M, NMIC10N, NcMIC10C) | Micronemes (18 kDa) (Yin et al. 2012) | T. gondii | (Yin et al. 2012) |
| rSubtilisin-like serin protease (N54, NcSUB1, NcSUB1t, NcSUB1tr) | Unknown (97, 87, 77, 67 and 64 kDa) (Louie et al. 1997) | T. fetus | (Louie et al. 1997) |