Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 3;198(11):4413–4424. doi: 10.4049/jimmunol.1601991

Figure 10. Chronic stimulation with SEB elicits autoantibody production and immune cell infiltration of multiple organs in CD4CD8 DKO mice.

Figure 10

Ten to twelve weeks-old male and female WT and DKO HLA-DQ8 transgenic mice subcutaneously implanted with 7-day mini osmotic pumps delivering either PBS or SEB (50 μg) were killed 7 days after implantation. Sera were tested for antinuclear antibodies using Hep-2 cells. Panels A and B represent the staining pattern with negative control (NC) and positive control (PC) sera provided along with the kit. Representative staining patterns with sera obtained from WT (panel C) and DKO (panel E) HLA-DQ8 transgenic mice implanted with PBS pumps. Representative staining patterns with sera obtained from WT (panel D) and DKO (panel F) HLA-DQ8 transgenic mice implanted with PBS pumps. The sera from WT and DKO HLA-DQ8 transgenic mice implanted with PBS or SEB pumps were also tested for the presence of antinuclear antibodies by ELISA. Each bar represents mean±SEM from 3-4 mice per group. * p<0.05 and NS – Not significant.