Figure 2. Splenic immune compartment in HLA-DR3 transgenic mice lacking both CD4 and CD8 coreceptors.
Splenic mononuclear cells collected from 6- to 8-week-old WT and DKO HLA-DR3 mice of either sex were counted and analyzed by flow cytometry following staining with indicated antibodies. Panel A represents total splenocyte counts in WT and DKO mice. Panels B and C depict the percentage and absolute numbers of various splenocyte subsets, respectively. The percentage of T cells expressing TCR Vβ6 and Vβ8 within the total lymphocyte gate (panel D) and specifically within the CD3 gate (panel E) are also given. Each bar represents mean±SE values from 6-8 mice/group. * p<0.05 and NS – Not significant.