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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 3;198(11):4413–4424. doi: 10.4049/jimmunol.1601991

Figure 4. Determining the efficacy of proliferation of splenic CD3+ T cells from HLA-DR3 DKO mice to staphylococcal enterotoxin B in comparison to WT HLA-DR3 mice in an adoptive transfer model.

Figure 4

CD3+ splenocytes isolated from 8-week-old WT and DKO HLA-DR3 mice of either sex were labeled with CFSE, mixed and adoptively transferred into NOD.SCID recipients along with CD3-depleted splenocytes harvested from HLA-DR3 WT mice as APCs. Recipient mice were challenged with PBS or SEB. Mice were killed 72-hours later and the CFSE levels in CD4/CD8+ and CD4/CD8- populations was determined by flow cytometry. (A) Representative dot plots of splenocytes obtained from PBS- or SEB-challenged recipient mice showing expression patterns of CFSE and CD4/CD8. (B) Representative histogram overlays depicting CFSE expression in splenocytes obtained from PBS- or SEB-challenged recipient mice gated on CD4/CD8 expression. (C) Representative histogram overlays depicting CFSE expression in splenocytes obtained from WT (left) or DKO (right) mice challenged with PBS or SEB. (D) Table represents % of cells within each peak. Representative data from 2 similar experiments is shown.