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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 3;198(11):4413–4424. doi: 10.4049/jimmunol.1601991

Figure 8. Chronic exposure to SEB causes multiple organ immunopathology and CD3+ T lymphocyte infiltration in liver.

Figure 8

Ten to twelve weeks-old WT and DKO HLA-DQ8 transgenic mice of either sex subcutaneously implanted with 7-day mini osmotic pumps delivering either PBS or SEB (50 μg) were killed 7 days after implantation. (i) Formalin-fixed, paraffin-embedded tissue sections from mice treated as above were stained with H&E and evaluated microscopically. Representative images are shown. Panels A, B and C – Respective lung, kidney and liver sections from WT HLA-DQ8 transgenic mice challenged with PBS. Panels D, E and F – Respective lung, kidney and liver sections from WT HLA-DQ8 transgenic mice challenged with SEB. Panels G, H and I – Respective lung, kidney and liver sections from DKO HLA-DQ8 transgenic mice challenged with PBS. Panels J, K and L – Respective lung, kidney and liver sections from DKO HLA-DQ8 transgenic mice challenged with SEB. (ii) Frozen liver sections from WT HLA-DQ8 transgenic mice challenged with PBS (panel M) or SEB (panel N) and liver sections from DKO HLA-DQ8 transgenic mice challenged with PBS (panel M) or SEB (panel N) were stained with FITC-conjugated anti-CD3 antibodies. Representative images from PBS and SEB treated mice are shown. (iii) Mean organ histopathology scores from WT and DKO HLA-DQ8 transgenic mice challenged with PBS or SEB was determined as described in methods section. Mean data from 4-5 mice/group. * p<0.05 when compared to respective PBS-treated mice and NS – Not significant.