Table 1. Paired case and control samples tested by quantitative real-time PCR, by age stratum and study site.
| Basse, The Gambia* | Bamako, Mali† | Manhiça, Mozambique† | Nyanza Province, Kenya* | Kolkata, India† | Mirzapur, Bangladesh* | Karachi (Bin Qasim Town), Pakistan* | Total | |
|---|---|---|---|---|---|---|---|---|
| Age stratum‡ | ||||||||
| 0–11 months | 300 (400) | 300 (727) | 267 (374) | 303 (673) | 300 (672) | 300 (550) | 300 (633) | 2070 |
| 12–23 months | 301 (455) | 300 (682) | 159 (195) | 300 (410) | 300 (588) | 300 (476) | 300 (399) | 1960 |
| 24–59 months | 165 (174) | 283 (624) | 95 (112) | 301 (393) | 300 (308) | 300 (368) | 226 (226) | 1670 |
|
| ||||||||
| Valid pairs analysed§ | 685 (894%) | 834 (94·5%) | 484 (92·9%) | 787 (87·1%) | 849 (94·3%) | 877 (974%) | 788 (954%) | 5304 (93·1%) |
|
| ||||||||
| Reproducibility (%)¶ | ||||||||
| MS2 | 6·0% | 5·2% | 6·0% | 6·8% | 5·9% | 6·2% | 5·8% | 67% |
| PhHV | 5·2% | 4·9% | 4·1% | 5·5% | 5·0% | 54% | 6·1% | 5·5% |
For each combination of age and site, we aimed to test 300 cases and first available matched controls; for sites with fewer than 300 cases enrolled, all available cases and first available matched controls were tested. PhHV=phocine herpes virus.
Extracted and tested on site
Extracted at University of Maryland, MD, USA, and tested at University of Virginia, VA, USA.
Numbers in parentheses are the number of cases in the original Global Enteric Multicenter Study.
Percentage of case-control pairs that were included in the analysis and did not exhibit extraction blank failures (139 pairs excluded, ranging from 0 to 7·0% by site), amplification failures (220 pairs excluded, ranging from 2·0% to 5·3% by site), or quantitative real-time PCR software flags (77 pairs excluded, ranging from 0·2% to 2·7% by site).
Measured by the coefficient variation of MS2 or PhHV Cqs on the clinical specimens.