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. 2017 Jun 15;13:177. doi: 10.1186/s12917-017-1081-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Phylogenetic trees of Leptospira spp. using (a) 16S rDNA and (b) secY sequences. The nucleotide sequences were aligned using Muscle, and the trees were generated using the neighbor-joining method (bootstrap: 1.000, model maximum composite likelihood). The clinical isolates from this work are indicated with arrows. Leptospira strains of the Hardjo serovar are indicated with arrowheads. Circles at nodes reflect the bootstrap support. An ancestral node without a circle indicates a bootstrap value below 50%. The bootstrap values of the nodes that group L. interrogans or L. kirschneri are located on these nodes in rectangles. Long branches are broken, and the real lengths are displayed on these branches in parenthesis. In both trees, orthologous sequences from Leptonema illini DSM 21528 were used as the outgroup