Table 2.
List of primers used for PCR detection, sequencing and qRT-PCR assays
| Gene target | Sequence (5′-3′) | Melting temp. (°C) | Amplicon length (bp) |
|---|---|---|---|
| 16S rDNA | F: ACT AAC GCT GGC GGC GCG R: TAC CCA CGC TTT CGT GCC |
56 | 1500 |
| secY (1) | F: GCG ATT CAG TTT AAT CCT G R: GCG ATT CAG TTT AAT CCT GC |
58 | 245 |
| ORF22 | F: ATT ATT GAG ACA GAG ATA R: AAG CGG AAC GGG ACG AAT |
56 | 561 |
| ORF36 | F: AGT CGG GGT TCG ATA CTG R: GGT CAG TCC TGT AAC TGC |
54 | 489 |
| IS1500a | F: TAC GGA GCA AGA ACG GTT R: AGA TCC GAT TCT TAT GAT TC |
56 | 143 |
| IS1533a | F: TTG TAA TCC CTG TGT TGT TT R: TTA AAT AGT CCA CTC CTC G |
56 | 130 |
| secY (2)a | F: CTG AAT CGC TGT ATA AAA GT R: GAA GGC TGG TAA ACA AAA G |
58 | 130 |
afor qRT-PCR