Fig. 3.

Pfs25-specific serum antibody response after primary immunization with an Ad vector expressing Pfs25. Mice (n = 3 per group) were immunized with Ad5 expressing Pfs25 (10¹⁰ particles) or recombinant yPfs25-alum (25 μg/mouse) on day 0, and serum samples were collected on day 21. a yPfs25-specific antibody titer. yPfs25-specific ELISA was performed and data depict pooled serum from 3 animals per group tested in triplicate in 3 independent ELISAs. The average ELISA unit and standard deviation are plotted. b yPfs25-specific avidity. Sera were added to a yPfs25-coated ELISA plate at a single dilution and then incubated with increasing concentrations of NaSCN. The remaining yPfs25-specific antibodies were detected with an HRP-conjugated anti-mouse IgG (Fc). The fraction of Ig bound was determined by dividing the optical density (OD) at each NaSCN concentration by the OD in the absence of NaSCN and the nonlinear best-fit line was plotted for each data set. c, d yPfs25-specific IgM and IgG subclass profile. yPfs25-specific ELISA was performed and the yPfs25-specific IgM and IgG subclasses were detected with HRP-conjugated specific antibodies. The average OD and standard deviation for each isotype/subclass (c) and the ratio of the O.D. for the IgG1 to the O.D.s of IgG2b and IgG3 (d) are shown