Table 1.

Primer sequences for recombineering

Primer name	Sequence
Primers for galk insertion
HVR1 galk forward	gtgccccaaatccttgcgaatgggatgaagctgctactgctcttgaaatacctgttgacaattaatcatcggca
HVR1 galk reverse	agaataaggcgcctgcccaaatacgtgagttttttgctgctcagcttgcttcagcactgtcctgctcctt
HVR5 galk forward	agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcacctgttgacaattaatcatcggca
HVR5 galk reverse	gtgtctggggtttctatatctacatcttcactgtacaataccactttaggtcagcactgtcctgctcctt
Primers for pfs25 epitope insertion
HVR1 DIII forward	gtgccccaaatccttgcgaatgggatgaagctgctactgctcttgaaataatctggatacatctaatcccgtgaagactggagtctgcagt
HVR1 DIII reverse	agaataaggcgcctgcccaaatacgtgagttttttgctgctcagcttgctcacaactgcagactccagtcttcacgggattagatgtatc
HVR5 pfsDII forward	agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcaattgatgggaacccagtgtcctacgcctgcaagtgtaat
HVR5 pfsDII reverse	gtgtctggggtttctatatctacatcttcactgtacaataccactttaggattacacttgcaggcgtaggacactgggttcccatcaat
HVR5 pfsDIII forward	agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcactggatacatctaatcccgtgaagactggagtctgcagt
HVR5 pfsDIII reverse	Gtgtctggggtttctatatctacatcttcactgtacaataccactttaggacaactgcagactccagtcttcacgggattagatgtatc