Fig. 5.

Characterization of McDXSs and McHDR. a Expression of champak 1-deoxy-D-xylulose 5- phosphate synthase (McDXSs) and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (McHDR) genes in leaves (L) and flowers (F). b Subcellular localization of McDXSs and McHDR. Auto, chlorophyll autofluorescence; YFP, YFP channel image; Merged, merged image of Auto and YFP; Light, light microscopy image. Bars, 20 μm. c and d Complementation assays of champak DXS isoforms (c) and HDR (d) using E.coli dxs ⁻ and MG1655 ara<>ispH mutants, respectively. For DXS complementation assay, E.coli cells containing Arabidopsis thaliana DXS (AtDXS1) and empty vector (EV) were used as a positive and negative control, respectively. For the HDR complementation assay, Arabidopsis thaliana HDR (AtHDR) was used as a positive control while empty vector (EV) and McDXS1 were used as negative controls