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. 2017 Jun 19;135(25):2505–2523. doi: 10.1161/CIRCULATIONAHA.116.025722

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© 2017 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 1. — Generating CD34⁺progenitors from human dermal fibroblasts. A, Schematic of converting human dermal fibroblasts into induced endothelial cells (iECs) and induced Erythroblasts (iErythroblasts) by sorted CD34⁺ progenitors from dedifferentiated fibroblasts (De-Diff-Fib) at day 7. B through D, Quantitative polymerase chain reaction (PCR) analysis of the mesodermal marker CD34 (B), pluripotency markers OCT4 (C), and Nanog (D) during adult fibroblast dedifferentiation from days 0 to 7 (n=4). E, Representative flow-cytometry plots of the mesodermal marker CD34⁺ in fibroblasts, adult De-Diff-Fib, and neonatal De-Diff-Fib. F, Quantification and statistical analysis of CD34⁺ cells in fibroblasts (n=3), adult De-Diff-Fib (n=6), and neonatal De-Diff-Fib (n=6) from flow cytometry. G through I, Representative flow-cytometry overlay plots of the pluripotency markers: human TRA-160 and TRA-180 in CD34⁺ progenitors (G) and iPSCs (H) and quantification of TRA-160 or TRA-180-positive cells (I) in CD34⁺ progenitors and iPSCs. Data are presented as mean±SE, *P<0.05, **P<0.01, ***P<0.001, compared with day 0 in B, C, and D, compared with fibroblasts in F, using repeated measures 1-way analysis of variance in B, C, and D and using 1-way analysis in F. N.D. indicates not detected in I.