Table 2. Primers and PCR conditions used for antibiotic resistance gene detection.
| Drug resistance genes | Positive control | Sequences (5′–3′) | Annealing temperature (°C) | Product size (bp) | References |
|---|---|---|---|---|---|
| Beta-lactams | |||||
| blaTEM | – | ttaactggcgaactacttac gtctatttcgttcatccata | 60 | 247 | Kozak et al. (2009) |
| blaSHV | – | aggattgactgccttttg atttgctgatttcgctcg | 60 | 393 | Kozak et al. (2009) |
| blaCMY-2 | – | gacagcctctttctccaca tggacacgaaggctacgta | 60 | 1,000 | Kozak et al. (2009) |
| Aminoglycosides | |||||
| aac(3)-IIa | – | cggaaggcaataacggag tcgaacaggtagcactgag | 60 | 740 | Soleimani et al. (2014) |
| aac(3)-IV | – | gtgtgctgctggtccacagc agttgacccagggctgtcgc | 60 | 627 | Maynard et al. (2004) |
| aadA | – | cccctggagagagcgagatt cgtggctggctcgaagatac | 60 | 152 | Our study |
| aadB | – | gaggagttggactatggatt cttcatcggcatagtaaaag | 60 | 208 | Kozak et al. (2009) |
| Quinolone | |||||
| qnrA | – | agaggatttctcacgccagg tgccaggcacagatcttgac | 60 | 580 | Cattoir et al. (2007) |
| qnrB | – | ggcattgaaattcgccactg tttgctgctcgccagtcgaa | 60 | 264 | Cattoir et al. (2007) |
| qnrS | – | gcaagttcattgaacagggt tctaaaccgtcgagttcggcg | 60 | 428 | Cattoir et al. (2007) |