Figure 4. c-Kit forms a molecular complex with the regulatory proteins TAK1, Src, and NLK in smooth muscle cells (SMC).

(A) Diagram illustrating the proposed molecular complex between c-Kit, TAK1, Src, and NLK in SMC and their inhibitory function on NF-κB transcriptional activity. (B–C) Co-immunoprecipitation experiments in control (Kit^+∕+) and c-Kit deficient (Kit^W∕W−v) SMC using anti-c-Kit (B) and anti-TAK1 antibodies (C) to pull down protein complexes. Molecular weight markers are shown on the right side of the gels, while antibodies used to detect eluted proteins are indicated on the left. IP, immunoprecipitation; IB, immunoblot.