Table 2. Sequences of hydrolysis probes used in evaluation of twelve genes of interest employed to investigate seasonal cold tolerance in D. ponderosae.

Gene		Probe sequence (5′–3′)
PK	Pyruvate kinase	56-TAMN-CAGCAGATCCTCCGCCTTCCAACAA-3BHQ_2
GP	Glycogen phosphorylase	56-ROXN-CAGCCCAAGCAATCCAGACGAGTTC-3BHQ_2
CS	Citrate synthase	56-FAM-CCACAGCAACGAAATAACACCACCA-3BHQ_1
6-PGL	6-phosphoglucolactonase	56-TAMN-ACACCTGCTCTCTGTTTCCTGGACA-3BHQ_2
G6PDH	Glucose-6-phosphate dehydrogenase	56-ROXN-AGCCTCGCCTGGTTGAACTCTAATC-3BHQ_2
G3PDH	Glycerol-3-phosphate dehydrogenase	56-TAMN-TCATCGTCCACCACCACCACTCG-3BHQ_2
FBP	Fructose-1,6-bisphosphatase	56-ROXN-AGCTACTCAATGCCATCCAGACTGC-3BHQ_2
ADH	Alcohol dehydrogenase	56-FAM-TCCAACACTCTCGACCACTCCAGC-3BHQ_1
TPI	Triosephosphate isomerase	56-TAMN-AAGTCCATCAGTCGCTACGCCAGTG-3BHQ_2
PEPCK	Phosphoenolpyruvate carboxykinase	56-ROXN-TTGACGAACTCCTCCGCCTCTTGC-3BHQ_2
GS	Glycogen synthase	56-TAMN-TCTTCAACACCGCCGAGGACCG-3BHQ_2
TAGL	Triacylglycerol lipase	56-ROXN-ACCCAAATAAGAGCCAGTGACGCCA-3BHQ_2