TABLE 1. Effects of pretreatment of DDT cells with p-DITC-ADAC on radioligand binding to cell membranes.
DDT cells were incubated with 1 μM p-DITC-ADAC or 1 μM CPA for 1 hr at 37° followed by six washes with HBSS. Cell membranes were then prepared, and the A1-AdoRs were determined by [3H]CPX (0.125–4 nM) binding to DDT cell membranes. The Kd and Bmax values were derived from Rosenthal plots. Data are the mean ± standard error of four or five separate determinations.
| [3H]CPX binding | Bmax | Kd |
|---|---|---|
| fmol/mg of protein | nM | |
| Pretreatment | ||
| Control | 275 ± 14 | 0.57 ± 0.06 |
| CPA | 262 ± 16 | 0.57 ± 0.02 |
| p-DITC-ADAC | 153 ± 11a | 0.56 ± 0.02 |
a
p < 0.0005 versus control.