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Effects of anaphylatoxins on gene expression of C3, C3aR, C5, and C5aR in ARPE-19 cells. Quantitative PCR analysis of the anaphylatoxin-dependent regulation of complement factor gene expression for C3 (upper left panel), C3aR (upper right panel), C5 (lower left panel), and C5aR (lower right panel). Anaphylatoxins were applied at concentrations of 52 nM (C3a) and 52 nM (C5a). Data are expressed as mean values + SEM, n = 3, *p < 0.05, ***p < 0.005 (Student’s t-test).
