TABLE 1.
Summary of H influenzae cell wall gene essentiality
| Gene | Locus | No. of Tn inserts/total no. of colonies screened | Essential in H. influenzae Rd |
|---|---|---|---|
| alr | HI1575 | 0/96 | Yes |
| amiB | HI0066 | 3/96a | No |
| dacA | HI0029 | 22/48 | No |
| dacB | HI1330 | 3/48 | No |
| ddlB | HI1140 | 0/96 | Yes |
| ftsI | HI1132 | 0/96 | Yes |
| glmS | HI0429 | 28/48 | No |
| glmU | HI0624 | 0/96 | Yes |
| glnA | HI0865 | 5/96a | No |
| lpp | HI1579 | 22/48 | No |
| mepA | HI0197 | 25/48 | No |
| mraY | HI1135 | 0/96 | Yes |
| mrsAb | HI1337 | No | |
| mrsAb | HI1463 | No | |
| mtgA | HI0831 | 5/96a | No |
| murB | HI0268 | 0/96 | Yes |
| murC | HI1139 | 0/96 | Yes |
| murD | HI1136 | 0/96 | Yes |
| murE | HI1133 | 0/96 | Yes |
| murF | HI1134 | 0/96 | Yes |
| murG | HI1138 | 0/96 | Yes |
| murI | HI1739 | 0/96 | Yes |
| murZ | HI1081 | 0/96 | Yes |
| nagA | HI0140 | 0/96 | Yes |
| pbp2 | HI0032 | 6/48 | No |
| pbpG | HI0364 | 25/48 | No |
| ponA | HI0440 | 36/96a | No |
| ponB | HI1725 | 16/48 | No |
| slt | HI0829 | 14/48 | No |
a
Exhibits a slow-growth phenotype compared to the wild type.
b
Individual gene copies are not essential; however, MrsA function is essential.