Fig. 4.

Partial alignment of CcmM amino acid sequences of 12 studied alkaliphiles with those of Synechococcus elongatus PCC 7942 (Syn7942), Synechocystis sp. PCC 6803 (Syn6803), Thermosynechococcus elongatus BP-1 (BP-1), and Nostoc sp. PCC 7120 (Noc7120) (GenBank accession no. BAA16773.1, Q03513.1, NP_681734, and BAB72822.1, respectively). The sequence order is based on the alignment. Boxes represent conserved regions of the N-terminal domain of CcmM that are assumed to be necessary for CA activity, according to Pena et al. [73]. Shaded cysteine amino acids showed essential residues participating in the disulfide bond in the C-termini of active CcmM protein. Asterisks indicate conserved amino acids inside such regions.