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. 2017 Jul;65:40–51. doi: 10.1016/j.ceca.2017.02.006

Fig. 6.

Fig. 6

Effect of Na+/Ca2+ exchange on GCaMP6f signals in Ca2+ free solutions.

(A) GCaMP6f fluorescence traces in response to blue excitation in 0 Ca2+ bath (1 mM EGTA) in dissociated ommatidia expressing GCaMP6f in wild-type, calx1 mutants lacking exchanger activity and in ninaE-calx flies over-expressing the exchanger. Traces are mean ± S.E.M. n = 6-8 ommatidia. (B) calx1 GCaMP6f trace (mean ± S.E.M. n = 6) replotted at high gain. (C) maximum ΔF/Fo values (after 2 s) from wild-type, calx1 and ninaE-calx backgrounds in both normal bath (Ca2+) and Ca2+ free bath (0 Ca2+) with normal Na+. (D) wild-type, calx1 and ninaE-calx Ca2+ free ΔF/F0 values replotted on log10 plot along with 0 Ca2+ 0 Na+ data (Cs+ substitution) from wild-type and ninaE-calx.