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. 2017 Jul;65:40–51. doi: 10.1016/j.ceca.2017.02.006

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — Effect of Na⁺/Ca²⁺ exchange on GCaMP6f signals in Ca²⁺ free solutions.

(A) GCaMP6f fluorescence traces in response to blue excitation in 0 Ca²⁺ bath (1 mM EGTA) in dissociated ommatidia expressing GCaMP6f in wild-type, calx¹ mutants lacking exchanger activity and in ninaE-calx flies over-expressing the exchanger. Traces are mean ± S.E.M. n = 6-8 ommatidia. (B) calx¹ GCaMP6f trace (mean ± S.E.M. n = 6) replotted at high gain. (C) maximum ΔF/F_o values (after 2 s) from wild-type, calx¹ and ninaE-calx backgrounds in both normal bath (Ca²⁺) and Ca²⁺ free bath (0 Ca²⁺) with normal Na⁺. (D) wild-type, calx¹ and ninaE-calx Ca²⁺ free ΔF/F₀ values replotted on log₁₀ plot along with 0 Ca²⁺ 0 Na⁺ data (Cs⁺ substitution) from wild-type and ninaE-calx.