FIG 2 .

CrzA translocates to the nucleus upon caspofungin exposure. (A) The expression of crzA, as determined by RT-qPCR, is induced in the presence of caspofungin. The wild-type strain was grown for 16 h at 37°C and transferred to increasing caspofungin concentrations for 60 min. All gene expression was normalized by the amount of β-tubulin (tubC). Standard deviations present the average from 3 independent biological repetitions (each with 2 technical repetitions). Statistical analysis was performed using a one-way ANOVA with Dunnett’s post hoc test compared to the control condition (*, P < 0.05). (B) Cellular localization of the CrzA::GFP strain, as determined by microscopy, when grown for 16 h at 30°C and after incubation in the presence of caspofungin (CSP), cyclosporine (Cyclo), or both cyclosporine and caspofungin. The percentage of CrzA::GFP nuclear localization is indicated for each condition and is based on counting between 300 and 600 nuclei in 50 to 100 hyphal germlings of biological triplicates. Bars, 5 μm.