Figure 3.

Circulating lipid profile does not correlate with microbiota encroachment in human. Colonic biopsies were collected during colonoscopy procedure and placed in methanol-Carnoy fixative solution, followed by confocal microscopy analysis of microbiota localization. (A) Distances of the closest bacteria to intestinal epithelial cells was measured in 5 high-powered fields per sample and plotted versus cholesterol concentration. Linear regression line was plotted and R² and P values were determined. (B) Distances of the closest bacteria to intestinal epithelial cells was measured in 5 high-powered fields per sample and plotted versus triglyceride concentration. Linear regression line was plotted and R² and P values were determined. (C) Distances of the closest bacteria to intestinal epithelial cells was measured in 5 high-powered fields per sample and plotted versus low-density lipoprotein concentration. Linear regression line was plotted and R² and P values were determined. n = 42; red dots represent subjects with diabetes. IEC, intestinal epithelial cells; LDL, low-density lipoprotein.