Table 1.

Molecular properties of the Blomia tropicalis allergens

Allergen	MW1, kDa	Protein function/family	PDB No.	Allergenicity test	IgE reactivity, %	Recombinant production/organism	References
Blo t 1	25	Cysteine protease	–	SPT	2 – 93	E. coli and P. pastoris	11, 28, 30
Blo t 2	13	Unknown	–	ELISA	18 – 57	E. coli	11, 34
Blo t 3	24	Trypsin	–	SPT/ELISA	9 – 57	E. coli	11, 35
Blo t 4	55	α-Amylase	–	SPT	1 – 63	P. pastoris	11, 29, 40, 41
Blo t 5	14	Unknown	2JMH 2JRK 2MEY	SPT	12 – 98	E. coli, P. pastoris, and Chinese hamster ovary cells	21 – 23, 40, 41
Blo t 6	26	Chymotrypsin	–	SPT	0 – 77	E. coli and P. pastoris	40, 41, 46
Blo t 8	28	Glutathione S-transferase	4Q5N	SPT	10 – 100	E. coli	11, 58, 59
Blo t 10	33	Tropomyosin	–	SPT/ELISA	10 – 38	E. coli	11, 55, 61
Blo t 11	102	Paramyosin	–	Dot blot	5 – 87	E. coli and Sf9 insect cells	25, 40, 65
Blo t 12	14	Unknown	2MFK	RAST	0 – 70	E. coli and P. pastoris	6, 40, 41, 68
Blo t 13	15	Fatty-acid-binding protein	–	RAST	7 – 11	E. coli and P. Pastoris	11, 27, 72, 74
Blo t 19	7	Antimicrobial peptide homolog	2MFJ	–	10	–	75
Blo t 21	11	Unknown	2LM9	SPT/ELISA	57 – 93	E. coli	11, 76

MW, molecular weight; E. coli, Escherichia coli; P. pastoris, Pichia pastoris.

¹Calculated using ProtParam [114]. Only the sequence of the mature chain was used for the calculations. The sequences used for the ProtParam analysis were selected according to the following criteria: (i) sequence published in PDB database (http://www.rcsb.org/pdb), and (ii) in case the first criteria was not fulfilled, the isoform 0.0101 sequence published in the International Union of Immunological Societies database was used.