Table 5. Diversity, frequency, and main molecular features of Cryptosporidium isolates at the GP60 and SSU rRNA loci in clinical samples, San Pedro Hospital, Logroño (La Rioja), 2015–2016.

Marker	Species	Family	Sub-genotype	No. isolates	Reference sequence	Stretch	SNPs	GenBank accession No.
GP60	C. hominis	Ib	IbA10G2	54	AY262031	24−857	None	KY499044
				1	AY262031	24−857	G216T	KY499045
				1	AY262031	24−857	A327G, A756G	KY499046
				1	AY262031	24−857	T425Aa	KY499047
				1	AY262031	24−857	C497Yb, C581Yc	KY499048
				1	AY262031	24−857	C579M	KY499049
	C. parvum	IIa	IIaA14G2R1	1	JF727773	6−858	T37Cd, C270Te, T480Gf, A486G, C529Tg	KY499050
			IIaA15G2R1	4	AY262034	43−872	None	KY499051
			IIaA16G2R1	1	AY262034	45−872	146_147insATC	KY499052
			IIdA17G1	1	LT556065	9−830	G277Ah	KY499053
				1	LT556065	9−830	G277Ah, T279A	KY499054
SSU	C. hominis	‒	‒	7	AF108865	567−978	None	KY499055
	C. parvum	‒	‒	4	AF112571	539−1,026	A646G, T649G, 686_689delTAAT, T693A	KY499056
	C. parvum	‒	‒	1	AF112571	533−1,030	A646G, T649G, 686_689delTAAT, T693A, C751A	KY499057
	C. parvum	‒	‒	1	AF112571	535−1,026	A646G, 647_649delATT, T663C, 686_689delTAAT	KY499058
	C. parvum	‒	‒	1	AF112571	573−997	A646G, T649G, 686_689delTAAT, C795Y	KY499059

GenBank accession numbers of representative sequences were provided. Novel genotypes were shown underlined. Point mutations inducing amino acid substitutions or stop codons were highlighted as superscript numbers indicating the amino acid change. SNP: single nucleotide polymorphism.

^ap.Leu142*.

^bp.Ser166Phe.

^cp.Ser194Phe.

^dp.Leu12Ser.

^ep.Gln90*.

^fp.Tyr160Asp.

^gp. Ser176Phe.

^hp.Ala93Thr.