Fig 2. Defective spermatogenesis and acrosome formation in Gorasp2 mutant mice.

(A) Sections of epididymis from control littermate and Gorasp2^-/- mice stained with hematoxylin and eosin. Note that most of the mutant lumens are empty and that some of them contain large round cells instead of differentiated spermatozoa (inserts). Scale bars: top panels, 100 μm; bottom panels, 50 μm; inserts, 10 μm. (B) Sections of testes from 35 days-old control and Gorasp2^-/- mice stained for the acrosomal protein SP56. Scale bar, 100 μm. (C) High magnification images of knock-out and control testes sections stained with anti-SP56 and peanut agglutinin (PNA). Note that SP56 staining is more restricted than PNA staining. Scale bar, 10 μm.