Fig 5. DIC inhibits cell viability and induces apoptosis in A2780 cells.

(A) A2780 cells were treated with increasing concentrations of DIC for 24 h, and cell viability was measured by the MTT assay. (B) After the indicated treatments for 24 h, A2780 cells were stained with annexin V and PI, and analyzed by flow cytometry. (C) The quantification of annexin V⁺PI⁺ apoptotic cells. (D) Upon treatment, the protein levels of caspase-3, cleaved caspase-3, PARP, and cleaved PARP in A2780 cells were examined by western blot, with representative western blot images presented on the left and the quantitation of protein expression relative to β-actin (internal control) presented on the right. Data are presented as the mean ± SE from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, when compared to nontreated control cells.