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. 2017 Jun 15;12(6):e0179492. doi: 10.1371/journal.pone.0179492

Fig 3. Inhibitory effect of HDL on hypoxia induced ROS production in H9c2 cells.

Fig 3

(A) Representative western blots. ‘Image J’ software was used to calculate the expression level of protein (B). ROS was examined by DCF-AM (10μM), DHE (5μM), and MitoSOX™ (5μM). Fluorescence intensity of cells was measured by flow cytometry. (C) Neonatal cardiomyocytes were treated with HDL (25–100μg/ml) for 2h, or NAC (500 μM), roteone (5 μM), and then incubated with 1%hypoxia for an additional 24h, and followed by 1h incubation with DHE (10μM). Fluorescence intensity of cells was measured by immunofluorescence microscopy (Olympus CKK53). Data showed the means ± SEM of 3 independent analyses.#P<0.05 comparison of control and hypoxia groups, *P<0.05 HDL/ NAC treated groups vs. hypoxia treatment.