Figure 4.

Effect of rhein on the expression levels of NF-κB p65, iNOS, and COX-2 in LPS-stimulated macrophages.

Notes: Representative Western blot images of NF-κB p65 (A), iNOS (B), and COX-2 (B) over β-actin in LPS-stimulated macrophages with or without different concentrations of rhein. The bar graph shows the quantitative analysis of densitometric data of NF-κB, iNOS, and COX-2. Each value represents the mean ± SEM (*P<0.01 vs LPS; analysis of variance).

Abbreviations: SEM, standard error of the mean; iNOS, inducible nitric oxide synthase; NF-κB, nuclear factor-κB; LPS, lipopolysaccharide.