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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Cell Metab. 2017 Mar 7;25(3):686–697. doi: 10.1016/j.cmet.2017.02.010

Figure 7. Acylceramides Accumulate in Liver Lipid Droplets of HFD Fed Mice.

Figure 7

(A) Liver sections of control diet or oleate HFD (4 weeks) fed mice were stained with H&E or Oil red O and visualized.

(B) Interaction of CerS6, ACSL5, and DGAT2 in liver lysates of control diet or oleate HFD (4 weeks) fed mice (n=3 for each group) was detected by IP with anti-CerS6 antibody followed by Western Blotting. SPT and ACER1 blotting was used as controls representing other ER resident proteins.

(C and D) Total ceramides and acylceramides were measured in livers of mice that were fed a control diet or oleate enriched HFD for four weeks using LC/MS. (E and F) Total ceramide and acylceramide levels were measured in lipid droplets from the livers of control diet or oleate enriched HFD (4 weeks) fed mice.

(G and H) Total acylceramide levels were measured in livers (G) and lipid droplets from the livers (H) of mice that were fed a control diet or oleate enriched HFD for 8 weeks with or without PF-06424439 treatment.

Results are expressed as means (n=4 for each group in 4 week study) (n=3 for each group in 8 week study) with error bars representing ± SEM. *p <0.05 and ** p< 0.01.