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. 2017 May 26;6:e27389. doi: 10.7554/eLife.27389

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© 2017, Wang et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) SEC-MALS profiles for BG505 SOSIP.664 Env trimer and complexes of Env trimer with BG1, PG16, and 8ANC195 Fabs. The absorbance at 280 nm (left y-axis) is plotted against elution volume from a Superdex 200 10/300 GL gel filtration column and overlaid with the molar mass determined for each peak (right y-axis). Predicted and calculated molecular masses are shown in the table. (B) Negative-stain single particle EM reconstructions BG1-Env-8ANC195, PG9-Env-8ANC195, and PG16-Env-8ANC195 complexes. EM density was fit with coordinates for the indicated Fabs and for BG505 Env trimer. (C) Density and coordinates from the BG1-Env portion of the BG1-Env-8ANC195 reconstruction (density for 8ANC195 Fabs removed) with coordinates for the indicated Fabs superimposed. The Env trimer portion from EM structures of complexes of PGT145-Env (PDB 5U1F), PG9-Env (panel B), or PG16-Env (panel B) were superimposed with the Env trimer portion of the BG1-Env-8ANC195 structure (panel B).

DOI: http://dx.doi.org/10.7554/eLife.27389.005

Figure 2—source data 1. Molar neutralization ratios (MNRs) for select bNAbs (MNR = [IC₅₀ Fab (nM)/IC₅₀ IgG (nM)].
The geometric mean MNR was calculated for each bNAb on a set of 25–30 HIV-1 strains with measured IC₅₀s values for both Fab and IgG forms. The measure of variation of the MNRs across strains is the geometric standard deviation, which is the exponential of the standard deviation of the logarithms of the individual MNRs.

DOI: http://dx.doi.org/10.7554/eLife.27389.006

elife-27389-fig2-data1.xlsx^{(51KB, xlsx)}

DOI: 10.7554/eLife.27389.006

Figure 2—figure supplement 1. — (A) SEC-MALS profiles for BG505 SOSIP.664 Env trimer and complexes of Env trimer with BG1, PG16, and 8ANC195 Fabs. The absorbance at 280 nm (left y-axis) is plotted against elution volume from a Superdex 200 10/300 GL gel filtration column and overlaid with the molar mass determined for each peak (right y-axis). Predicted and calculated molecular masses are shown in the table. (B) Negative-stain single particle EM reconstructions BG1-Env-8ANC195, PG9-Env-8ANC195, and PG16-Env-8ANC195 complexes. EM density was fit with coordinates for the indicated Fabs and for BG505 Env trimer. (C) Density and coordinates from the BG1-Env portion of the BG1-Env-8ANC195 reconstruction (density for 8ANC195 Fabs removed) with coordinates for the indicated Fabs superimposed. The Env trimer portion from EM structures of complexes of PGT145-Env (PDB 5U1F), PG9-Env (panel B), or PG16-Env (panel B) were superimposed with the Env trimer portion of the BG1-Env-8ANC195 structure (panel B).

DOI: http://dx.doi.org/10.7554/eLife.27389.005

Figure 2—source data 1. Molar neutralization ratios (MNRs) for select bNAbs (MNR = [IC₅₀ Fab (nM)/IC₅₀ IgG (nM)].
The geometric mean MNR was calculated for each bNAb on a set of 25–30 HIV-1 strains with measured IC₅₀s values for both Fab and IgG forms. The measure of variation of the MNRs across strains is the geometric standard deviation, which is the exponential of the standard deviation of the logarithms of the individual MNRs.

DOI: http://dx.doi.org/10.7554/eLife.27389.006

elife-27389-fig2-data1.xlsx^{(51KB, xlsx)}

DOI: 10.7554/eLife.27389.006