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Apoptosis in the nucleus pulposus (NP) detected by TUNEL staining. (A) Apoptotic cells were visualized using fluorescence microscopy and light microscopy (200 ×, 400 ×). (B) Quantification of the apoptotic incidence between groups. Apoptosis varied significantly at each time point after puncture. **P < 0.01, values represent the mean ± SD of six discs from three rabbits.
