Table 2.

Primer sequences used for site-directed mutagenesis.

Primer name	Sequence (5′-3′)
PIM1 K5R For	TTC AAT GCT CTT GTC CAG AAT CAA CTC GCT TTG CCC
PIM1 K5R Rev	GGG CAA GCG AGT TGA TTC TGG ACA AGA GCA TTG AA
PIM1 K24R For	GGG CGC CAG CCT GGT GGC GTG CA
PIM1 K24R Rev	TGC ACG CCA CCA GGC TGG CGC CC
PIM1 K29, 31R For	AGG GGC TCC CTC TCC CTG CCG GGC GCC
PIM1 K29, 31R Rev	GGC GCC CGG CAG GGA GAG GGA GCC CCT
PIM1 K67R For	CTC CAC GTG TCT GAT GGC CAC CGG CAA GTT
PIM1 K67R Rev	AAC TTG CCG GTG GCC ATC AGA CAC GTG GAG
PIM1 K67M For	GTC CTT CTC CAC GTG CAT GAT GGC CAC CGG CA
PIM1 K67M Rev	TGC CGG TGG CCA TCA TGC ACG TGG AGA AGG AC
PIM1 K71R For	AAA TCC GGT CCC TCT CCA CGT GTT TGA TGG CCA
PIM1 K71R Rev	TGG CCA TCA AAC ACG TGG AGA GGG ACC GGA TTT
PIM1 K94, 95 R For	CCG AGC TCA CCC TCC TCA GCA GGA CCA CTT CCA TGG
PIM1 K94, 95 R Rev	CCA TGG AAG TGG TCC TGC TGA GGA GGG TGA GCT CGG
PIM1 K169R For	GTG CTC CAC CGC GAC ATC AGG GAC GAA AAC ATC
PIM1 K169R Rev	GAT GTT TTC GTC CCT GAT GTC GCG GTG GAG CAC
PIM1 E171A For	CCG CGA CAT CAA GGA CGC AAA CAT CCT TAT CGA CC
PIM1 E171A Rev	GGT CGA TAA GGA TGT TTG CGT CCT TGA TGT CGC GG
PIM1 E171Q For	GAG GTC GAT AAG GAT GTT CTG GTC CTT GAT GTC GCG GTG
PIM1 E171Q Rev	CAC CGC GAC ATC AAG GAC CAG AAC ATC CTT ATC GAC CTC
PIM1 E181A For	CGA TGA GCT TGA GCG CGC CGC GAT TGA GG
PIM1 E181A Rev	CCT CAA TCG CGG CGC GCT CAA GCT CAT CG
PIM1 K183R For	GAA GTC GAT GAG CCT GAG CTC GCC GCG ATT G
PIM1 K183R Rev	CAA TCG CGG CGA GCT CAG GCT CAT CGA CTT C
PIM1 K194R For	GAC GGT GTC CCT GAG CAG CGC CCC C
PIM1 K194R Rev	GGG GGC GCT GCT CAG GGA CAC CGT C
PIM1 K313R For	GAA AGG CTG CTA TCT GCT GGG CCC CGG
PIM1 K313R Rev	CCG GGG CCC AGC AGA TAG CAG CCT TTC