Figure 1.

Talin-1 R3 domain destabilization by mutagenesis. (a) Schematic illustration of wild-type talin-1 and the locations of the mechanically destabilizing point mutations in the R3 subdomain in mutants 1S, 2S, 3S and 4S. (b) A cartoon model (PDB ID: 2L7A¹⁴) of mouse talin-1 R3 domain. The four mutated residues are shown as stick models colored in red and indicated with red labels. The pulling vectors in the SMD simulations are marked with a dashed black line and respective amino acids are indicated with dark coloring. The two vinculin binding helices in the R3 subdomain are colored in blue. (c) Talin-1 R3 domain helix boundaries and sequence conservation across 13 animal species (Human gi6739602, Mouse gi227116327, Rat gi189181726, Hamster gi344251776, Naked mole rat gi351707040, Threeshrew gi444729903, Bat gi432110771, Flying fox gi431902812, Cattle gi296484714, Turtle gi465952424, Quail gi667665823, Chicken gi45383127, Zebrafish gi57222259). The positions of the two VBS helices and four destabilizing point mutations are colored in blue and red, respectively.