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. 2017 Jun 15;7:3575. doi: 10.1038/s41598-017-03909-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure. 7 — Akt inhibitor IV prevents glycine-induced functional recovery of ischemic stroke animals. For all the three experiments, Glycine (100 µg/100 g, icv) was administered at 3 h following I/R. Akt inhibitor IV (100 µM, 2 µl, icv) is injected with MK-801 and strychnine at 30 min before glycine injection. (A) Animals treated with glycine (100 µg/100 g. icv) has lower scores of mNSS test at day 7 and 14 compared with I/R + Stry + MK + IV + Gly group (n = 10; ANOVA test, *P < 0.05, vs. I/R + Stry + MK + IV + Gly). (B) Animals treated with glycine has lower scores of beam-walking test at day 3, 7 and 14 compared with I/R + Stry + MK + IV + Gly group (n = 10; ANOVA test, *P < 0.05, vs. I/R + Stry + MK + IV + Gly). (C) Animals treated with glycine (100 µg/100 g, icv) treatment has higher ratio in MST test at day 7 and 14 compared with I/R + Stry + MK + IV + Gly group (n = 10; ANOVA test, *P < 0.05, vs. I/R + Stry + MK + IV + Gly). Stry: Strychnine; Gly: glycine; MK: MK-801.