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. 2017 Jun 15;7:3578. doi: 10.1038/s41598-017-03874-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fractionation of retinal cells by EGFP expression from pHes1-EGFP transgenic mouse retina. (A,B,C) RNA-seq using purified EGFP positive (P) and negative (N) cells of retina from pHes1-EGFP mouse at P1 and P4 was done. RNA-seq values of genes related to Müller glia (A,B) or photoreceptor (C) are shown. (D,E) Unsupervised K-means clustering of all genes on the RNA-seq to generate 4 clusters was done (D), and the distribution of gene expression of the clusters 0, 1, 2, and 3 in pHes1-EGFP positive (P) and negative (N) populations are shown (E). Numbers in parentheses are gene number in each cluster. Statistical analysis was done by student’s t test, and effect size of the results was examined by Pearson’s correlation coefficient (r) and indicated in the figure. N, negative; P, positive.