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. 2017 Jun 15;7:3578. doi: 10.1038/s41598-017-03874-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H3K27me3 levels of pHes1_EPC-specific genes loci in pHes1-EPC and pHes1-ENC. H3K27me3 levels of representative genes of each cluster in pHes1-EPC and pHes1-ENC at P1 and P4. ChIP using anti-H3K27me3 antibody was done using extracts of purified pHes1-EPC and pHes1-ENC from mouse retina at P1 and P4. qPCR was done using representative genes in each cluster. The experiments were done three times, and values are average of three samples with standard deviation. p value; **<0.01, *<0.05, or n.s. >0.05 was calculated by Student’s t-test.