Figure 6.

Characterization of genes upregulated in P4 but not in P1 in pHes- EGFP positive cell populations. Genes of pHes1-EPG at P4 but not included in pHes1-EPG at P1 were sub-clustered to C1 to C7 by using their values of H3K4me3 and H3K27me3 ChIP-seq of Cd73_P and Cd73_N fractions¹³, and distribution of gene expression levels (RNA-seq) in each cluster are shown by box plot (A). (B–D) Box plots of distribution of H3K4me3 (ChIP-seq, B), H3K27me3 (ChIP-seq, C), and transcripts level (RNA-seq, D) of genes in each sub-cluster are shown. ChIP-seqs and RNA-seq were performed by using Cd73_P and Cd73_N retinal sub-fractions at P2, P5, and P8¹³. (E) Percentage of genes categorized to transcription factors by IPA in total number of genes in the clusters is shown. (F–I) Distribution of gene expression levels in pHes1-EPC or pHes1-ENC (F), or in Cd73_P and Cd73_N (G). Levels of H3K4me3 (H) and H3K27me3 (I) of Cd73_P and Cd73_N fractions of the transcription factors in each cluster are shown.