Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 15;7:3614. doi: 10.1038/s41598-017-03972-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of ectopic miR-200 overexpression in UCS cell lines. (A) miR-200c overexpression in SNU-685 and JHUCS-1 cell lines. Bar graphs represent relative miR-200c expression. Data are normalized to non-targeting controls (NTC). (B) Relative mRNA expression of EMT markers. miR-200c overexpression data are normalized to NTC. Changes in SNU-685 vimentin mRNA expression were not statistically significant. (C) Protein expression of EMT markers by immunoblotting. Anti-β-actin serves as a loading control. (D) Morphologic changes in miR-200c-overexpressed UCS cells. Rows represent lower (top) and higher (bottom) magnifications. The spindle-shaped, elongated control cells are outlined in blue. The cobblestone-shaped miR-200c-overexpressed cells are outlined in orange. (E) Relative cell viability measured by VisionBlue™ assay 2 days (SNU-685) and 3 days (JHUCS-1) after seeding. Data are normalized to NTC. (F) Relative proliferation measured by cell counts. Data are normalized to Day 0. (G) Relative adhesion, migration and invasion. There were no statistically significant differences in migration or invasion between miR-200c-overexpressed and control SNU-685 cells. All graphical data are reported as mean ± SD. *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001; paired sample t-test.