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. 2017 Jun 15;7:3614. doi: 10.1038/s41598-017-03972-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential expression of EMT/MET-related genes in EAC-miR-200 depleted and UCS-miR-200 overexpressed cells. (A) Heatmap of RNA expression for 26 EMT/MET-related genes. Relative RNA expression of miR-200b/c-depleted vs scramble-treated Ishikawa and MFE-280 cells, and miR-200c-overexpressed vs non-targeting control treated SNU-685 and JHUCS-1 cells, are shown. Increased and decreased expression are depicted in red and blue, respectively. The top 17 genes are known to have increased expression in the mesenchymal phenotype, while the bottom 9 genes are known to have increased expression in the epithelial phenotype. Relative expression is calculated by log2 fold-change (B).