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. 2017 Jun 15;7:3615. doi: 10.1038/s41598-017-03851-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endothelialization formation of the explanted grafts at 1 month after implantation. (A) The lumen surface of explanted grafts was observed by SEM. (B) The endothelial coverage of grafts was observed by En face immunostaining using CD31 antibody (Red arrows: blood flow direction). (C) Endothelialization was analyzed by immunofluorescence staining of longitudinal sections of grafts using CD31 antibody (L: lumen; Red arrows: suture site). (D) The endothelial coverage at 1 and 3 months was calculated based on the longitudinal sections with CD31 staining. *p < 0.05.