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PCR primers for virulence gene detection.
| No. | Primers | Sequence (5’→3’) | bp | Function |
|---|---|---|---|---|
| 1 | asa1-F | CACGCTATTACGAACTATGA | 375 | Surface adhesion |
| asa1-R | TAAGAAAGAACATCACCACGA | substances | ||
| 2 | ace-F | GGAATGACCGAGAACGATGGC | 616 | Surface adhesion |
| ace-R | GCTTGATGTTGGCCTGCTTCCG | proteins | ||
| 3 | cylA-F | ACTCGGGGATTGATAGGC | 688 | Hemolysin |
| cylA-R | GCTGCTAAAGCTGCGCTT | |||
| 4 | gelE-F | TATGACAATGCTTTTTGGGAT | 213 | Gelatinase |
| gelE-R | AGATGCACCCGAAATAATATA | |||
| 5 | hyl-F | ACAGAAGAGCTGCAGGAAATG | 276 | Hyaluronidase |
| hyl-R | GACTGACGTCCAAGTTTCCAA |
