Table 1.
Primer sequences.
| Forward | Reverse | Product size (bp) | |
|---|---|---|---|
|
Primers for SLA class I amplicon generation | |||
| SLA class I (short) | Exon 2-forward: 5′-CGTGGACGACACGCAGTTC-3′ | Exon 3-reverse: 5′-TCCAGYAGCGCAGGTCCTC-3′ | 320 |
| SLA class I (long) | Exon 2-forward: 5′-CGTGGACGACACGCAGTTC-3′ | Exon 4-reverse: 5′-AGGTCAGAGCTGGGGRGG-3′ | 510 |
|
Primers for sequencing of novel SLA class I sequences (NS) | |||
| Complete exon 2 | Exon 1-forward-SLA-1/3: 5′-GCCCTCTTCCTGCTGCTG-3′ | Inner reverse for NS#1–8, 11, 12: 5′-GTAATCGGCGCCGTCGTAGG-3′ | 420 (SLA-1 or -3) |
| Exon 1-forward-SLA-2: 5′-AGCCATCCTCATTCTGCTGT-3′ | Inner reverse for NS#9: 5′-GTAATCCGCGCCGTCGTAGG-3′ | 421 (SLA-2) | |
| Inner reverse for NS#10: 5′-GTAATCGGCGCCGTCGTAGC-3′ | |||
| Complete exon 3 | Inner forward for NS#1, 4, 5, 6, 9, 10: 5′-CGCACAGACTTACCGAGTG-3′ | Exon 4-reverse: 5′-AGGTCAGAGCTGGGGRGG-3′ | 381 |
| Inner forward for NS#2: 5′-CGCACAGAATTACCGAGTG -3′ | |||
| Inner forward for NS#3,7,8,12: 5′-CGCACAGATTAACCGAGTG-3′ | |||
| Inner forward for NS#11: 5′-CGCAGAGATTAACCGAGTG-3′ | |||
Primer sequences for generation of the 320 and 510 nt amplicon as well as for sequencing of the complete exon 2 and exon 3 of novel SLA class I sequences are listed. All primers were 5′-barcoded to allow tracing of the fragments to individual pigs. Y = C or T, R = A or G.