Figure 6.

Biofilm formation of V. alginolyticus supplemented with exogenous 3-oxo-C₁₀-HSL at 28°C (A) Quantification of biofilm formed by strains N°24 and N°40 cultured for 36 h at 28°C using the semi-quantitative adhesion test. (B) Representative 3D-reconstructed biofilm structure of strain N°24 using CLSM. (C) Representative 3D-reconstructed biofilm structure of strain N°40 using CLSM. The “Standardized biofilm” corresponds to the average value of 3 replicates; the “error bar” represents standard deviation of 3 replicates; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; the “N.C” (negative control) refers to the strains cultured in 2216E broth without exogenous 3-oxo-C₁₀-HSL for 36 h.