Table 4.
Quantitative analysis of the biofilm matrices of V. alginolyticus strains at 28°C.
| Strains and culture conditions | BioMass (mg/cm3) | Average thickness (μm) | Max thickness (μm) | Roughness coefficient | Microcolonies at substrate | |
|---|---|---|---|---|---|---|
| N°24 | N.C | 0.046 ± 0.024 | 3.736 ± 1.247 | 8.500 ± 2.121 | 0.491 ± 0.358 | 2.500 ± 0.707 |
| 1 μmol/L 3-oxo-C10-HSL | ND | ND | ND | ND | ND | |
| 10 μmol/L 3-oxo-C10-HSL | 2.949 ± 0.086** | 6.090 ± 2.083* | 9.000 ± 1.414 | 1.842 ± 0.138** | 2.500 ± 0.707 | |
| 20 μmol/L 3-oxo-C10-HSL | 3.273 ± 0.127** | 4.090 ± 0.628 | 11.000 ± 1.414 | 1.297 ± 0.646** | 6.000 ± 1.414 | |
| N°40 | N.C | 9.984 ± 1.677 | 12.987 ± 2.124 | 21.000 ± 2.646 | 0.187 ± 0.025 | 36.733 ± 4.162 |
| 1 μmol/L 3-oxo-C10-HSL | 15.167 ± 2.262* | 17.808 ± 2.556* | 21.500 ± 2.364* | 0.234 ± 0.074 | 43.667 ± 3.786 | |
Biofilm matrix index values were analyzed using COMSTAT software and shown as mean of 3 replicates with standard deviation. The “N.C” (negative control) refers to the strains cultured in 2216E broth without exogenous 3-oxo-C10-HSL for 36 h; the “ND” for not detected;
*
P < 0.05;
**
P < 0.01.