Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 15;7:3584. doi: 10.1038/s41598-017-03616-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analyses of fibrotic reactions in end-stage OA synovial tissues and fibroblast cultures. (A) Histochemical images of synovial hyperplasia, hyperangiogenesis (arrows), and fibrotic tissue positive for Masson’s trichrome stain in the end-stage OA group. Panels in the low power field images indicate areas of interest for high power field images. (B) OA group showed increases in synovial membrane thickness, vessel formation, and fibrosis tissue area. (C) OA synovial tissues exhibited high miR-29a but not b, c expression. (D) The OA cartilage displayed weak Safranin-O staining. Synovial fibroblasts and chondrocytes (arrows) in the OA group displayed weak miR-29a transcripts. Data (mean ± standard error) are calculated from 20 patient with end-stage OA and 8 participants with non-OA injury and analyzed by a Wilcoxon test. miR-29a transfection reduced expression of (E) collagen III, TGF-β1, PLOD2, TIMP1, and ADAM12 in concomitant with (F) low expression of MMP9, MMP13 and ADAMTS5 in synovial fibroblasts. Loss of miR-29a signaling increased fibrogenic factor and proteinase expression in cell cultures. Synovial fibroblast experiments in triplicate were repeated 3 times. All investigations (mean ± standard error) of synovial fibroblasts were analyzed by a parametric analysis of variance (ANOVA) and a Bonferroni post-hoc test. Asterisks (*) stands for significant difference between groups.