Skip to main content
. 2017 Jun 16;11:169. doi: 10.3389/fncel.2017.00169

Figure 1.

Figure 1

Serping1 affects neuronal stem cell proliferation. (A,B) Embryonic brains were in utero electroporated with control shRNA or Serping1 shRNA at E13 and at E14 were treated with IdU for 30 min. The brains were cryosectioned and immunostained with anti-IdU antibodies. GFP labeled the electroporated cells. IMARIS software was used to count the total GFP-positive cells and the double GFP- and IdU-positive cells within slices of the same size (230 μm in length). Double-positive cells are marked with white asterisks in the GFP panel. The relative proportion of double-positive cells to the total number of GFP-positive cells was calculated (B, Student t-test, n = 5, *p < 0.05). (C,D) Brains of E13 Serping1 KO and littermate WT were treated with IdU for 30 min. The brains were cryosectioned and immunostained with anti-IdU and anti-TBR2 antibodies. The number of IdU-positive, TBR2-positive, double positive, IdU-positive TBR2-negative cells was counted (D) in identical areas of the cortices (200 μm in length). Welch's t-test, n = 6, *p < 0.05, **p < 0.01. The scale bars are 50 μm.