Effect of farnesol on CSH of C. albicans biofilms. Different farnesol concentrations (0, 1, 10, and 100 μM) were added to C. albicans cells 1 h after attachment, and the cells were incubated under conditions favorable for biofilm growth. CSH was estimated by the water-hydrocarbon two-phase assay. Mean relative CSH values and standard deviations for three separate experiments are shown. Statistically significant differences between farnesol-treated biofilms and biofilms formed in the absence of farnesol were determined by Student's t test (*, P < 0.01).