FIGURE 7.

Effects of UA treatment on the expression of inflammatory cytokines (A) and NF-κB activation (B). Non-irradiated control did not receive any drug treatment or gamma radiation. 6.4 Gy group mice only exposed to 6.4 Gy gamma radiation without any drug treatment. UA+6.4 Gy group mice received ip injection of UA (25 mg/kg bw) for 14 days, followed by exposure to 6.4 Gy gamma irradiation at day 15 and same treatment till day 30. (A) The expression level of inflammatory cytokines TNF-α and IL-6 was evaluated in plasma by ELISA. (B) Expression of p65, phospho-p65, and β-actin was evaluated by western blot in colon tissue of mice. The image was captured and analyzed by Bio-Rad Gel Doc system. The results were calculated and expressed as fold change relative to control. Data were expressed as mean ± SEM. Comparisons between groups were analyzed by One-Way ANOVA followed by Tukey’s post hoc test. ^∗p < 0.05 vs non-irradiated. #p < 0.05 vs irradiated and no drug treatment.