. 2017 Jun 16;8:1111. doi: 10.3389/fmicb.2017.01111

Table 2.

Effects of nisin and monensin addition on the alpha diversity measurements of ruminal bacteria at 3% dissimilarity level.

Item	NC	MON	NI1	NI5	SEM	P-value
# of sequences	26,999	29,585	29,205	31,958	2,255	0.51
Coverage (%)	99.6	99.6	99.6	99.5	0.04	0.57
OTU	665^a	612^b	644^ab	613^b	13.8	0.05
ACE	747	716	722	725	9.2	0.15
Chao 1	748	712	726	721	10.7	0.16
Shannon index	4.40^a	3.71^d	4.27^b	4.03^c	0.036	<0.01
Simpson index	0.039^c	0.067^a	0.041^c	0.050^b	0.002	<0.01

NC = negative control (no additives); MON = monensin, 5 μM; NI1 = nisin, 1 μM; NI5 = nisin, 5 μM; OTU = operational taxonomic units; ACE = abundance-based coverage estimator.

^a-dMeans within a row with different superscripts differ (P < 0.05).