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. 2017 Jun 9;8:15754. doi: 10.1038/ncomms15754

Figure 2. CoMIC is potentiated by inhibition of Drp1, but does not depend on Drp1.

Figure 2

(a) Time-lapse images of the mitochondrial matrix and neuronal processes in DN-Drp1-expressing neurons (DIV4). Arrowheads indicate sites of mitochondrial bulging. Scale bars, 10 and 5 μm in the lower and higher magnification images, respectively. (b) Probability of CoMIC in elongated mitochondria (>5 μm) as compared with control (70 mitochondria) and DN-Drp1-expressing neurons (114 mitochondria). Error bar indicate s.e. (c) Representative kymographic analysis of a mitochondrion showing CoMIC in a DN-Drp1-expressing neuron. Arrow heads indicate conserved bulging site on two CoMIC-showing time points (1, 2). (d) Quantification of the total duration, frequency and mean interval of CoMIC in control and DN-Drp1-expressing neurons (***P<0.01 by Mann–Whitney rank sum test). (e) Straightened images of DsRed-mito showing minimal bulging (cyan signal) and maximal bulging (red signal) in control and DN-Drp1-expressing neurons. (f) Bulging ratio in foci during CoMIC in control and DN-Drp1-expressing neurons (***P<0.01 by Mann–Whitney rank sum test). (g) CoMIC in Drp1 knockout mouse embryonic fibroblasts. The time-lapse panels are enlarged in the green and blue boxes. Arrowheads indicate the sites of mitochondrial bulging. Scale bars, 10 and 5 μm in the lower and higher magnification images, respectively.